Defining the role of APC in the mitotic spindle checkpoint in vivo APC Biology Diagrams Bernard, P., Hardwick, K. & Javerzat, J. P. Fission yeast bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis. J. Cell The spindle checkpoint, also known as the metaphase-to-anaphase transition, the spindle assembly checkpoint (SAC), the metaphase checkpoint, or the mitotic checkpoint, is a cell cycle checkpoint during metaphase of mitosis or meiosis that prevents the separation of the duplicated chromosomes Abstract. In cells containing disrupted spindles, the spindle assembly checkpoint arrests the cell cycle in metaphase. The budding uninhibited by benzimidazole (Bub) 1, mitotic arrest-deficient (Mad) 1, and Mad2 proteins promote this checkpoint through sustained inhibition of the anaphase-promoting complex/cyclosome.
Abstract. The mitotic checkpoint is a specialized signal transduction pathway that contributes to the fidelity of chromosome segregation. The signaling of the checkpoint originates from defective kinetochore-microtubule interactions and leads to formation of the mitotic checkpoint complex (MCC), a highly potent inhibitor of the Anaphase Promoting Complex/Cyclosome (APC/C)โthe E3 ubiquitin
Principles and dynamics of spindle assembly checkpoint signalling Biology Diagrams
Taylor, S. S. et al. Kinetochore localisation and phosphorylation of the mitotic checkpoint components Bub1 and BubR1 are differentially regulated by spindle events in human cells. J. Cell Sci The mitotic spindle checkpoint. Gary J. Gorbsky. Biomedical Research Center, Room 266, 975 N.E. 10th St., University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA If the spindle checkpoint is defective (left pathway) then chromatid separation can occur before all the chromosomes are aligned at metaphase. The
The spindle checkpoint is an evolutionarily conserved mitotic regulatory mechanism that ensures that anaphase is not attempted until chromosomes are properly aligned on the spindle. Two different cell-cycle transitions must be inhibited by the spindle checkpoint to arrest cells at metaphase and prevent mitotic exit. The checkpoint proteins interact in ways that are more complex than was Spindle Checkpoint. The spindle checkpoint ensures that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle. This safeguard prevents premature chromatid separation by monitoring kinetochore-microtubule interactions. Checkpoint proteins such as Mad1, Mad2, Bub1, and BubR1 delay cell cycle progression until
